Hsp27/HSPB1 Rabbit Polyclonal Antibody

SKU: orb215991

描述

The Hsp27/HSPB1 Rabbit Polyclonal Antibody is an unconjugated antibody raised in rabbit. It is immunogen affinity purified and suitable for use in FC, ICC, IF, IHC, IHC-Fr, IP, and WB applications. This antibody targets the Heat shock protein beta-1 in Human samples.

研究领域

Cancer Biology, Cardiovascular Research, Cell Biology, Neuroscience, Protein Biochemistry, Signal Transduction

图片与验证

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已测应用	FC, ICC, IF, IHC, IHC-Fr, IP, WB
稀释范围	Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, - Immunocytochemistry/Immunofluorescence, 2μg/ml, Human, - Immunoprecipitation, 0.5-2 μg/ml, Human Flow Cytometry (Fixed), 1-3μg/mlx10^6 cells, Human
反应性	Human
应用说明	For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.

关键属性

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抗体类型	Primary Antibody
宿主	Rabbit
克隆性	Polyclonal
同种型	Rabbit IgG
免疫原	E.coli-derived human Hsp27 recombinant protein (Position: M1-K205). Human Hsp27 shares 83% amino acid (aa) sequence identity with mouse Hsp27.
靶点	Heat shock protein beta-1
分子量	27 kDa
纯化	Immunogen affinity purified.
偶联	Unconjugated

储存与操作

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储存	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
形态/外观	Lyophilized
缓冲液/防腐剂	Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
浓度	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
有效期	12 months from date of receipt.
免责声明	For research use only

别名

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Heat shock protein beta-1; HspB1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27; HSPB1; HSP27, HSP28

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质量保证

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