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Biorbyt offers custom peptide synthesis services for sequences up to 100 amino acids in length. Standard custom synthesis yields range from 5 mg to 50 mg with purities up to >95%, alongside flexible bulk production options to support large-scale research initiatives.
Custom Modification Services
Terminal & Side-Chain Modifications
Comprehensive N-terminal, C-terminal, and specific side-chain modifications tailored for enhanced stability, reactivity, or target binding.
D-Amino Acid & Isotope Labeling
Precision incorporation of D-amino acids and stable-isotope labeled residues for metabolic tracing and proteolytic resistance studies.
Biotinylation
Targeted biotin conjugation at N/C-terminals or specific lysine residues for high-affinity streptavidin capture and assay detection.
Fluorescent Labeling
Covalent attachment of diverse fluorophores (FITC, FAM, Cy dyes, Rhodamine) for cell-imaging, FRET, and binding assays.
Post-Translational Modifications
Accurate synthesis of modified peptides incorporating phosphorylation (Ser/Thr/Tyr), acetylation, and methylation patterns.
Multiple Disulfide Bridges
Regioselective folding techniques to construct complex peptides containing single or multiple intramolecular disulfide bonds.
Glycosylation
Specialized incorporation of glycan moieties (N-linked and O-linked sugars) to mimic native eukaryotic glycoprotein structures.
PEGylation
Site-specific attachment of polyethylene glycol (PEG) chains to significantly extend plasma half-life and reduce immunogenicity.
Production Techniques & Quality Control
Peptides are delivered in lyophilized form at guaranteed purities up to >95%. Following resin cleavage, HPLC purification, and drying, synthetic peptides naturally contain bound water and counter-ions (such as TFA). These counter-ions do not impact biochemical activity, though net peptide content typically accounts for >70% of total mass.
Analytical Evaluation Techniques
- Analytical HPLC and Chiral HPLC testing
- Mass Spectrometry (MS) identity verification
- Nuclear Magnetic Resonance (NMR) structural analysis
- Thin-Layer Chromatography (TLC) screening
- High-voltage electrophoresis assessment
- Melting point & optical rotation measurement
- Complete analytical raw data archived and available upon request
Additional Services Upon Request
- Amino Acid Analysis (AAA): Precise quantification to determine exact peptide composition and net peptide content.
- Microanalysis: Elemental analytical methods utilized for exact net peptide content determination.
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