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Polyclonal antibody production involves immunizing an animal with a specific antigen, triggering the immune system to generate a diverse pool of antibodies targeting multiple epitopes. These antibodies are harvested from serum or plasma, yielding a multi-epitope recognizing antibody mixture with broad binding affinity.
Biorbyt provides a reliable and diverse range of custom polyclonal antibody production services, offering multiple host species options (rabbit, goat, chicken, rat) and immunogen choices to accommodate custom research requirements.
Advantages of Biorbyt's Polyclonal Antibodies
Broad Binding Specificity
Polyclonal antibodies recognize multiple epitopes on the target antigen, providing broader binding specificity and higher tolerance to minor antigen conformational changes.
High Sensitivity
Due to their ability to bind multiple epitopes simultaneously, polyclonal antibodies offer high signal amplification, enabling detection of low-abundance target proteins.
Fast & Cost-Effective
Polyclonal antibody development workflows offer rapid turnaround timelines and economical project costs compared to monoclonal cell line production.
Application Versatility
Ideal for detecting complex, modified, or denatured protein targets across a wide spectrum of research applications including Western Blot, IHC, and ELISA.
Natural Immune Response
Polyclonal antibody pools mimic the natural in vivo immune response, providing a rich antibody repertoire capable of robust target recognition.
Cross-Reactivity Potential
Conserved epitope recognition allows polyclonal antibodies to detect target protein isoforms across closely related species when broad detection is required.
Service Process & Deliverables
Peptide Immunogen
Lead Time: 12 – 15 WeeksRecombinant Protein
Lead Time: 15 – 17 WeeksNative Protein
Lead Time: 12 – 15 WeeksWorkflow Process
- Antigen preparation & quality check
- Animal immunization schedule
- Serum titer detection & monitoring
- Antigen affinity or Protein A/G chromatography purification
- Western Blot (WB) validation against target antigen
Key Deliverables
- Comprehensive Quality Control (QC) report for immunogen target
- Guaranteed ELISA titer threshold of 1:10,000
- Guaranteed Western Blot (WB) positive validation against antigen
- 1 mL pre-immune serum and 2 mL purified antiserum
- Guaranteed antibody purity of >90% by SDS-PAGE analysis
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