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Embark on a seamless journey from antigen preparation to antibody validation with our Rabbit Monoclonal Antibody (mAb) Development Service. Whether starting from antigen design or leveraging our pre-validated B cell library, Biorbyt offers decision-grade expertise in generating high-affinity rabbit monoclonal antibodies tailored to complex research and diagnostic targets.
Our proprietary Single B platform unlocks the potential to produce over 10,000 rabbit monoclonal antibodies from a single immunized animal, yielding exceptional clonal diversity. With over 5,000 pre-validated IgG sequences targeting 500+ therapeutic and diagnostic targets, our proven approach ensures high efficiency, rapid turnaround, and robust batch-to-batch reproducibility.
Service Highlights
Efficient Single B Platform
Utilizing our proprietary Single B isolation platform, we efficiently isolate, screen, and culture antigen-reactive B cells from immunized rabbits to recover rare, high-affinity binders.
Pre-Validated B Cell Libraries
Access Biorbyt’s extensive pre-validated B cell libraries targeting 500+ research targets to discover ideal clones directly, reducing development timelines, costs, and project risks.
Mammalian Cell Display Optimization
Selected mAb candidates can be further engineered using our mammalian cell display system to optimize binding affinity, expression yield, structural stability, and cross-reactivity profiles.
Development Workflow
A breakdown of our end-to-end development phases from immunogen design to final recombinant mAb release:
| Stage | Service Details | Deliverables | Estimated Time |
|---|---|---|---|
| 1. Immunogen Preparation | Plasmid construction, recombinant protein expression, or peptide synthesis for immunogen production in mammalian systems. | Sequence-confirmed construct; Purified immunogen target | 7 – 10 Weeks |
| 2. Animal Immunization | Immunize at least two rabbits per target using optimized booster protocols to elicit a strong, specific immune response. | ELISA test data on crude serum titers | 5 – 8 Weeks |
| 3. B Cell Isolation & Amplification | PBMC isolation, cryopreservation, antigen-positive single B cell enrichment, and single B cell culture expansion. | ELISA test data on B cell culture supernatants | 2 – 3 Weeks |
| 4. IgG Gene Cloning & Validation | Recombinant IgG gene cloning, small-scale expression, and preliminary validation; 1–2 positive clones selected for full-length sequencing. | Full-length sequencing report of verified clones with ELISA data | 3 – 4 Weeks |
| 5. mAb Production & QC Validation | Recombinant antibody expression in CHO/HEK293 cells, protein A/G purification, and comprehensive quality control testing. | 1 mg purified recombinant mAb with complete COA & QC report | 3 – 4 Weeks |
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