This is a specialized kit for one-step RT-qPCR based on the probe method. Reverse transcription and qPCR reactions are completed in the same tube, which makes the operation simple and fast. This product utilizes a new type of heat-resistant, high extension capacity Evo Super M-MLV Reverse transcriptase, integrated mutant Biorbyt Super Taq The superior performance of HS polymerase enables efficient cDNA synthesis and stable qPCR amplification in a short period of time. The product is widely used, with high impurity tolerance, and can amplify genes of different complexity, while it can be used for single or multiplexed detection. It is suitable for the detection of DNA/RNA and other trace viruses. The dUTP/UNG anti-contamination system is introduced in this product. dUTP replaces dTTP in the PCR reaction, and the UNG enzyme selectively hydrolyzes dU-containing DNA strands without affecting non-dU-containing DNA strands, removing dU-containing contaminating templates introduced during the preparation of the PCR reaction system, thus effectively preventing the generation of false-positive results in PCR; At the same time, monoclonal antibody that can inhibit the activity of DNA Polymerase at room temperature is added to this product, which can effectively inhibit non-specific amplification, improve the sensitivity of the reaction and enhance the accuracy of the results.
图片与验证
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应用说明
1. This is a one-step RT-qPCR reaction kit, which can complete the reverse transcription and qPCR reaction in a single tube, simplifying the operation, improving the efficiency, and effectively reducing the possibility of contamination caused by multiple operations. 2. This product adopts a new type of heat-resistant (up to 55℃), strong elongation ability. Evo Super M-MLV Reverse transcriptase and mutant Biorbyt Super Taq HS, paired with a carefully optimized reaction system, is characterized by high specificity and amplification efficiency. 3. The product is highly sensitive and can stably detect single-digit copy templates, and can perform single or multiple detection. 4. This product can perform DNA/RNA co-detection. 5. dUTP/UNG is a contamination prevention system that removes contamination of dU-containing PCR products, thus preventing false-positive PCR results and improving the accuracy of the results.
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