5-Propargylamino-dCTP-Cy5

SKU: orb533141

描述

5-Propargylamino-dCTP-Cy5 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incor-porated as substitute for its natural counterpart dCTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of cytidine. Recommended Propargylamino-dCTP-Cy5/dCTP ratio for PCR and Nick Translation: 30-50% Propargylamino-dCTP-Cy5/70-50% dCTP.

图片与验证

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Item 1 of 2

应用说明	Incorporation into DNA/cDNA by: PCR with Taqpolymerasein-house data; Nick Translation with DNAse I/DNA Polymerase Iin-house data. Spectroscopic Propertie: λexc 649 nm, λem 670 nm, ε 250.0 L mmol-1 cm-1 (Tris-HCl pH 7.5).

关键属性

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MW	Theoretical MW: 1159.01 g/mol (free acid); Detected MW: 1158.23 g/mol (free acid)
纯度	≥ 95% (HPLC)
分子式	C₄₅H₅₇N₆O₂₀P₃S₂
SMILES	N(CC#Cc1cn(c(=O)nc1N)[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@H](C1)O)C(=O)CCCCCN1c2ccc(S(=O)(=O)O)cc2C(C)(C)/C/1=C\C=C\C=C\C1=*(c2ccc(cc2C1(C)C)S(=O)(=O)[O-])CC

客户验证数据

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反应性分布

最新实验

4 Results

稀释

样本

摘要

DNA nick labeling for optical mapping

Bacteria

100 nM

Genomic DNA, plasmid DNA, PCR-amplified DNA fragments, and synthetic DNA constructs

5-Propargylamino-dCTP-Cy5 is used in a DNA...

Fluorescent DNA labeling

Human

Synthetic DNA oligonucleotides, enzymatically generated DNA microscaffolds, and live mammalian cells,

5-Propargylamino-dCTP-Cy5 is used as a fluorescently...

Fluorescent nucleotide incorporation assay

In vitro system

Synthetic single-stranded DNA oligonucleotides and purified TdT enzymes

5-Propargylamino-dCTP-Cy5 is used as a fluorescently...

Fluorescent labeling for 5-hydroxymethylcytosine detection

Mouse

Genomic DNA extracted from various tissues

5-Propargylamino-dCTP-Cy5 is used as a fluorescent...

储存与操作

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储存	store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible.
有效期	12 months from date of receipt.
免责声明	For research use only

别名

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5-Propargylamino-2'-deoxycytidine-5'-triphosphate, labeled with Cy5, Triethylammonium salt

相似产品

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5-Propargylamino-dCTP-Cy5 [orb1733849]
纯度：
≥ 95% (HPLC)
MW:
Theoretical MW: 1159.01 g/mol (free acid); Detected MW: 1158.23 g/mol (free acid)
分子式：
C₄₅H₅₇N₆O₂₀P₃S₂
可选规格： 10 μl

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实验方案信息

查看更多实验方案

纯度：	≥ 95% (HPLC)
MW:	Theoretical MW: 1159.01 g/mol (free acid); Detected MW: 1158.23 g/mol (free acid)
分子式：	C₄₅H₅₇N₆O₂₀P₃S₂