6X His Tag Antibody Biotin Conjugated

SKU: orb344543

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描述

6X His Tag antibody (Biotin)

图片与验证

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Item 1 of 2

已测应用	ELISA, IHC, WB
稀释范围	ELISA: 1:50,000 - 1:175,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
应用说明	Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used to identify fusion proteins containing the 6X His epitope. The antibody recognizes the His tag fused either to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and His-containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.

关键属性

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抗体类型	Primary Antibody
宿主	Mouse
克隆性	Monoclonal
同种型	IgG1
克隆号	33D10.D2.G8
免疫原	This antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
纯度	Anti-6X HIS Epitope Tag was purified from concentrated tissue culture supernate by Protein A chromatography and is directed against the 6X His motif and is useful in determining its presence in various assays. This monoclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag. To date, this antibody has reacted with all His tagged proteins so far tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins. The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy-termini of targeted proteins in transfected or transformed cells.
偶联	Biotin

储存与操作

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储存	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
形态/外观	Lyophilized
缓冲液/防腐剂	Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
浓度	1.0 mg/mL
有效期	12 months from date of receipt.
免责声明	For research use only

别名

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mouse anti-6X His Tag biotin conjugated Antibody, biotin conjugated mouse anti-6X His Tag Antibody, anti-HIS, HIS Antibody, 6X His Tag Antibody, HHHHHH epitope tag antibody

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