AICAR

SKU: orb1308527

特色

描述

AICAR (Acadesine) is a small molecule AMPK activator widely used in metabolic, cancer, and aging research. Its roles in regulating cellular energy and muscle function have been validated in both in vitro and in vivo experimental models.

研究领域

Cell Biology, Stem Cell & Developmental Biology

图片与验证

−

关键属性

−

CAS 号	2627-69-2
MW	258.2
纯度	>98%
分子式	C₉H₁₄N₄O₅
SMILES	NC(=O)c1ncn([C@@H]2O[C@H](CO)[C@@H](O)[C@H]2O)c1N
靶点	Kinase
溶解性	Soluble in DMSO (up to 15 mg/ml) or in Water (up to 15 mg/ml).

生物活性

−

靶点 IC50	Autophagy (rat hepatocytes):0.3 mM
体内	METHODS: To study the anti-tumor activity of AICAR, AICAR was subcutaneously injected (300 mg/kg/ day) into xenograft tumor mice derived from the H1975 cell line. RESULTS: AICAR significantly inhibited tumor growth, and the tumor weight decreased by 47% (p<0.05). The expression level of Ki-67 in tumor tissues treated with AICAR decreased, indicating a reduction in cell proliferation, while the expression levels of DNA damage markers γ-H2AX and p21Cip1 increased. METHODS: To study the anti-tumor activity of AICAR, xenograft mice of MG63 and KHOS osteosarcoma cell lines were administered with AICAR (450 mg/kg/ day). RESULTS: AICAR treatment significantly inhibited tumor growth, and the tumor volume decreased by 14.1% and 35.4% respectively (p<0.05). Mitochondrial proliferation and apoptotic activities increased in tumor tissues treated with AICAR.
体外	METHODS: CCRF-CEM, HeLa, and L1210 cells were treated with different concentrations of AICAR for 3 days. particle counting analysis was used to evaluate the inhibition of cell growth. RESULTS: AICAR inhibited the growth of CCRF-CEM cells (IC50=210 μM) and HeLa cells (IC50=165 μM), but did not inhibit the growth of L1210 cells (IC50≥ 250 μM). METHODS: HCT-116 and HEK293 cells were treated with AICAR for 48 hours, and cytotoxicity was detected by the MTT method. RESULTS: AICAR is non-toxic to HCT-116 (EC50> 100 μM) and HEK293 (EC50> 100 μM) cells. METHODS: K562 and K-562R cells were treated with AICAR for 48 hours, and the cytotoxicity was detected by the XTT method. RESULTS: AICAR showed cytotoxicity to K562 (IC50=800 μM) and K-562R (IC50=800 μM). METHODS: K562 cells were treated with AICAR for 48 hours, and the anti-leukemia activity was detected by the XTT method. RESULTS: AICAR exhibited anti-leukemia activity against K562 (IC50=800 μM). METHODS: MDA-MB-231 and RCC4 cells were treated with AICAR for 48 hours, and the anti-tumor activity was detected by the XTT method. RESULTS: AICAR exhibited anti-tumor activity against MDA-MB-231 cells (IC50=1000 μM) and RCC4 cells (IC50=250 μM). METHODS: MDA-MB-453 and SK-BR-3 cells were treated with AICAR for 4 days, and their antiproliferative activity was detected by the Celltiter-glo luminescent cell viability assay. RESULTS: AICAR exhibited anti-tumor activity against MDA-MB-453 cells (IC50=1700 μM) and SK-BR-3 cells (IC50=180 μM). METHODS: HepG2 cells were treated with AICAR (0.1-1.0 mM) for 12, 24 and 48 hours, and the protein levels were detected by western blot. RESULTS: The expression levels of IR-β in 0.25, 0.5 and 1.0 mM AICAR significantly decreased to 50%, 53% and 46% of the control at 48 h.
细胞研究	Acadesine is added to K562 cell lines or primary cells (103 CD34+ cells/mL) growing in semisolid methyl cellulose medium. MethoCult H4100 or H4236 are used for cell lines and primary CD34+ cells respectively. Colonies are detected after 10 days of culture by adding 1 mg/mL of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reagent and are scored by Image J quantification software. (Only for Reference)

储存与操作

−

储存	-20°C
有效期	12 months from date of receipt.
免责声明	For research use only

别名

−

Acadesine

相似产品

−

Item 1 of 2
Mouse anti Human ATIC Antibody [orb109164]
应用：
IHC, WB
反应性：
Frog, Human, Rat
种属/宿主：
Mouse
克隆性：
Monoclonal
偶联：
Unconjugated
可选规格： 200 μg
Item 1 of 4
ATIC Antibody [orb21308]
应用：
ELISA, IF, IHC
反应性：
Human
种属/宿主：
Rabbit
克隆性：
Polyclonal
偶联：
Unconjugated
可选规格： 100 μg, 50 μg
Item 1 of 3
ATIC Rabbit Polyclonal Antibody [orb763105]
应用：
ELISA, FC, WB
反应性：
Human, Mouse, Rat
种属/宿主：
Rabbit
克隆性：
Polyclonal
偶联：
Unconjugated
可选规格： 100 μg
Item 1 of 2
ATIC Antibody [orb521879]
应用：
ELISA, IHC, WB
反应性：
Human, Mouse, Rat
种属/宿主：
Rabbit
克隆性：
Polyclonal
偶联：
Unconjugated
可选规格： 100 μl, 50 μl
Item 1 of 1
ZTP [orb63207]
纯度：
≥ 95% (HPLC)
MW:
Theoretical MW: 498.17 g/mol (free acid); Detected MW: 498.00 g/mol (free acid)
分子式：
C₉H₁₇N₄O₁₄P₃
可选规格： 5 x 50 μl

质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.

化合物标识

PubChem CID

17513 ↗

关键属性

−

暂无计算属性。

实验方案信息

查看更多实验方案

应用：	IHC, WB
反应性：	Frog, Human, Rat
种属/宿主：	Mouse
克隆性：	Monoclonal
偶联：	Unconjugated

应用：	ELISA, IF, IHC
反应性：	Human
种属/宿主：	Rabbit
克隆性：	Polyclonal
偶联：	Unconjugated

应用：	ELISA, FC, WB
反应性：	Human, Mouse, Rat
种属/宿主：	Rabbit
克隆性：	Polyclonal
偶联：	Unconjugated

应用：	ELISA, IHC, WB
反应性：	Human, Mouse, Rat
种属/宿主：	Rabbit
克隆性：	Polyclonal
偶联：	Unconjugated

纯度：	≥ 95% (HPLC)
MW:	Theoretical MW: 498.17 g/mol (free acid); Detected MW: 498.00 g/mol (free acid)
分子式：	C₉H₁₇N₄O₁₄P₃

AICAR

描述

研究领域

图片与验证

关键属性

生物活性

储存与操作

别名

相似产品

Mouse anti Human ATIC Antibody [orb109164]

ATIC Antibody [orb21308]

ATIC Rabbit Polyclonal Antibody [orb763105]

ATIC Antibody [orb521879]

ZTP [orb63207]

质量保证

化合物标识

关键属性

实验方案信息

可选规格