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TUBA1B Antibody

SKU: orb344426

描述

Tubulin alpha antibody

图片与验证

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已测应用ELISA, IF, IHC, Multiplex Assay, WB
稀释范围ELISA: 1:300,000, IHC: 2.5 µg/mL, IF: 0.1 µg/mL, WB: 1:1,000
反应性Human
应用说明
Anti-Tubulin Antibody has been tested for use in ELISA, immunohistochemistry, immunofluorescence microscopy and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~50 kDa in size corresponding to alpha tubulin by western blotting in most cell lysates or extracts.

关键属性

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抗体类型Primary Antibody
宿主Mouse
克隆性Monoclonal
同种型IgG1
克隆号17H11.F10
免疫原Anti-Tubulin Loading Control Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues near the C terminal end of human alpha tubulin protein.
靶点TUBA1B
纯度Anti-Tubulin Loading Control Antibody was purified by Protein A chromatography. This Loading Control antibody is directed against alpha tubulin. A BLAST analysis was used to suggest antibody reactivity with alpha tubulin from a wide range of organisms, including avian, mammalian aquatic, parasitic and alga sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of alpha tubulin. Such broad reactivity makes this antibody useful as an excellent loading control.
偶联Unconjugated

储存与操作

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储存Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
形态/外观Liquid (sterile filtered)
缓冲液/防腐剂Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
浓度1.0 mg/mL
有效期12 months from date of receipt.
干冰运输请注意:本产品需要干冰运输,将收取干冰附加费。
免责声明For research use only

别名

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mouse anti-Alpha-Tubulin Antibody, Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1, TUBA1B, tubulin loading control

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质量保证

质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.

UniProt 详情

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暂无 UniProt 数据

NCBI 参考序列

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相关登录号
基因转录本和蛋白产物的整理参考序列
RefSeq17986283

实验方案信息

WB
免疫印迹(WB)
查看实验方案
IHC
免疫组化
查看实验方案
IF
免疫荧光
查看实验方案
ELISA
酶联免疫吸附测定(ELISA)
查看实验方案

可选规格

请在下方选择规格

25 μl
免费二抗(20 µl)0/0

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