Alternative Block ELISA Blocking Buffer, Synthetic

SKU: orb623179

描述

ICT’s Alternative Block ELISA Blocking Buffer provides superior background-blocking performance to eliminate interference and minimize background noise on ELISA formats without the use of conventional cross-reactive protein additives.

图片与验证

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应用说明

1. Allow product to equilibrate to room temperature (25°C) prior to use. 2. Add 100 µL to each well. When reacting with peroxidase, a soluble blue reaction product develops that can be read at 370 nm or in a range of 620-650 nm. The reaction product is stable for 1 hour. 3. The reaction can be stopped using an appropriate stop solution, such as Stop Solution for TMB Substrates (STOPT), for endpoint assays. STOPT changes the chromogen from blue to yellow and the absorbance to 450 nm. For best results, sample absorbance should be monitored and read before values exceed 2.0 OD units. STOPT can increase the absorbance up to 3-fold, so the reaction should be stopped when absorbance values reach approximately 0.7 OD units.

关键属性

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储存与操作

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储存	2-8°C
浓度	1 X
有效期	12 months from date of receipt.
免责声明	For research use only

相似产品

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Alternative Block ELISA Blocking Buffer, Synthetic [orb699913]
可选规格： 500 ml
Alternative Block ELISA Blocking Buffer, Synthetic [orb1821200]
可选规格： 100 ml

质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.

实验方案信息

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