CD122 Rat Monoclonal Antibody (FITC)

SKU: orb1293873

描述

The CD122 Antibody-FITC labeled is suitable for FC, IF. It is a Monoclonal, FITC conjugated antibody which raised against Rat T cell line expressing mouse CD122 ( IL-2Rβ). Purification: The antibody was purified by affinity chromatography.

研究领域

Immunology & Inflammation

图片与验证

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已测应用	FC, IF
稀释范围	10 μl / assay
反应性	Mouse
应用说明	1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

关键属性

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抗体类型	Primary Antibody
宿主	Rat
克隆性	Monoclonal
同种型	Rat IgG2a kappa
克隆号	B7J6
免疫原	Rat T cell line expressing mouse CD122 ( IL-2Rβ)
靶点	IL2RB
纯化	The antibody was purified by affinity chromatography.
偶联	FITC

储存与操作

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储存	Shipped and store at 4°C for one year. Do not freeze.
形态/外观	Liquid
缓冲液/防腐剂	PBS, pH 7.3, 0.2% rAlbumin, and 0.02% sodium azide.
有效期	12 months from date of receipt.
免责声明	For research use only

别名

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Interleukin-2 receptor subunit beta; IL-2 receptor subunit beta; IL-2R subunit beta; IL-2RB; High affinity IL-2 receptor subunit beta; p70-75; p75; CD122

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Quick Database Links

Gene Symbol

IL2RB

UniProt

P16297

NCBI Gene ID

16185

UniProt 详情

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暂无 UniProt 数据

NCBI 基因详情

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暂无 NCBI 基因数据

实验方案信息

流式细胞术

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