CD16/CD32 Rat Monoclonal Antibody (PE/Cy5.5)

SKU: orb1293581

描述

The CD16/CD32 Antibody-PE/Cy5.5 labeled is suitable for FC, IF. It is a Monoclonal, PE/Cy5.5 conjugated antibody which raised against Sorted pre-B cells. Purification: The antibody was purified by affinity chromatography.

研究领域

Immunology & Inflammation

图片与验证

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已测应用	FC, IF
稀释范围	10 μl / assay
反应性	Mouse
应用说明	1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

关键属性

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抗体类型	Primary Antibody
宿主	Rat
克隆性	Monoclonal
同种型	Rat IgG2a lambda
克隆号	B15
免疫原	Sorted pre-B cells
靶点	FCGR2B; FCGR3A
纯化	The antibody was purified by affinity chromatography.
偶联	PE/Cy5.5

储存与操作

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储存	Shipped and store at 4°C for one year. Do not freeze.
形态/外观	Liquid
缓冲液/防腐剂	PBS, pH 7.3, and 0.02% sodium azide.
有效期	12 months from date of receipt.
免责声明	For research use only

别名

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CD32; FCG2; IGFR2; Low affinity immunoglobulin gamma Fc region receptor II-b; IgG Fc receptor II-b; CDw32; Fc-gamma RII-b; Fc-gamma-RIIb; FcRII-b; CD32

质量保证

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常用数据库链接

基因符号

FCGR2B; FCGR3A

UniProt

P08101, P08508

NCBI 基因 ID

14131, 14130

UniProt 详情

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暂无 UniProt 数据

NCBI 基因详情

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暂无 NCBI 基因数据

实验方案信息

流式细胞术

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