CD25 Mouse Monoclonal Antibody (PerCP/Cy5.5)

SKU: orb1293491

描述

The CD25 Antibody-PerCP/Cy5.5 labeled is suitable for FC, IF. It is a Monoclonal, PerCP/Cy5.5 conjugated antibody which raised against Native purified human CD25.. Purification: The antibody was purified by affinity chromatography.

研究领域

Immunology & Inflammation

图片与验证

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已测应用	FC, IF
稀释范围	10 μl / assay
反应性	Human
应用说明	1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

关键属性

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抗体类型	Primary Antibody
宿主	Mouse
克隆性	Monoclonal
同种型	Mouse IgG1 kappa
克隆号	B365X35
免疫原	Native purified human CD25.
靶点	IL2RA
纯化	The antibody was purified by affinity chromatography.
偶联	PerCP/Cy5.5

储存与操作

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储存	Shipped and store at 4°C for one year. Do not freeze.
形态/外观	Liquid
缓冲液/防腐剂	PBS, pH 7.3, 0.2% rAlbumin, and 0.02% sodium azide.
有效期	12 months from date of receipt.
免责声明	For research use only

别名

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Interleukin-2 receptor subunit alpha; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA; TAC antigen; p55; CD antigen CD25

相似产品

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IL2RA/CD25 Mouse Monoclonal Antibody (PerCP-Cy5.5) [orb2284240]
应用：
FC
反应性：
Human
种属/宿主：
Mouse
克隆性：
Monoclonal
偶联：
PerCP/Cy5.5
可选规格： 100 μl
Human CD25 Mouse Monoclonal Antibody (PerCP-Cy5.5) [orb1184634]
应用：
FC, ICC
反应性：
Human
种属/宿主：
Mouse
克隆性：
Monoclonal
偶联：
PerCP/Cy5.5
可选规格： 25 T, 50 T, 100 T
CD25/IL-2 Receptor alpha Mouse Monoclonal Antibody (PerCP/Cy5.5) [orb2710303]
应用：
FC
反应性：
Human
种属/宿主：
Mouse
克隆性：
Monoclonal
偶联：
Unconjugated
可选规格： 100 T

质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.

常用数据库链接

基因符号

IL2RA

UniProt

P01589

NCBI 基因 ID

3559

UniProt 详情

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暂无 UniProt 数据

NCBI 基因详情

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暂无 NCBI 基因数据

实验方案信息

流式细胞术

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