CD45 Mouse Monoclonal Antibody (PE/Cy5)

SKU: orb1293637

描述

The CD45 Antibody-PE/Cy5 labeled is suitable for FC, IF. It is a Monoclonal, PE/Cy5 conjugated antibody which raised against Native purified human CD45.. Purification: The antibody was purified by affinity chromatography.

研究领域

Immunology & Inflammation

图片与验证

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已测应用	FC, IF
稀释范围	10 μl / assay
反应性	Human
应用说明	1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

关键属性

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抗体类型	Primary Antibody
宿主	Mouse
克隆性	Monoclonal
同种型	Mouse IgG2a
克隆号	B1X6
免疫原	Native purified human CD45.
靶点	PTPRC
纯化	The antibody was purified by affinity chromatography.
偶联	PE/Cy5

储存与操作

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储存	Shipped and store at 4°C for one year. Do not freeze.
形态/外观	Liquid
缓冲液/防腐剂	PBS, pH 7.3, 0.2% rAlbumin, and 0.02% sodium azide.
有效期	12 months from date of receipt.
免责声明	For research use only

别名

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CD45; Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen; L-CA; T200; CD antigen CD45

相似产品

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CD45 Rat Monoclonal Antibody (PE/Cy5) [orb1293610]
应用：
FC, IF
反应性：
Mouse
种属/宿主：
Rat
克隆性：
Monoclonal
偶联：
PE/Cy5
可选规格： 25 assay, 100 assay
CD45RA Mouse Monoclonal Antibody (PE/Cy5) [orb1293636]
应用：
FC, IF
反应性：
Human
种属/宿主：
Mouse
克隆性：
Monoclonal
偶联：
PE/Cy5
可选规格： 25 assay, 100 assay
CD45RA Mouse mAb, PE-Cy5 conjugated [orb3163580]
应用：
IF
反应性：
Human
种属/宿主：
Mouse
克隆性：
Monoclonal
偶联：
PE/Cy5
可选规格： 100 μl
CD45RO Mouse mAb, PE-Cy5 conjugated [orb3163522]
应用：
IF
反应性：
Human
种属/宿主：
Mouse
克隆性：
Monoclonal
偶联：
PE/Cy5
可选规格： 100 μl
CD45 Mouse mAb, PE-Cy5 conjugated [orb3163607]
应用：
FC
反应性：
Human
种属/宿主：
Mouse
克隆性：
Monoclonal
偶联：
PE/Cy5
可选规格： 100 μl

质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.

常用数据库链接

基因符号

PTPRC

UniProt

P08575

NCBI 基因 ID

5788

UniProt 详情

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暂无 UniProt 数据

NCBI 基因详情

−

暂无 NCBI 基因数据

实验方案信息

流式细胞术

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免疫荧光

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应用：	FC, IF
反应性：	Mouse
种属/宿主：	Rat
克隆性：	Monoclonal
偶联：	PE/Cy5

CD45 Mouse Monoclonal Antibody (PE/Cy5)

描述

研究领域

图片与验证

关键属性

储存与操作

别名

相似产品

CD45 Rat Monoclonal Antibody (PE/Cy5) [orb1293610]

CD45RA Mouse Monoclonal Antibody (PE/Cy5) [orb1293636]

CD45RA Mouse mAb, PE-Cy5 conjugated [orb3163580]

CD45RO Mouse mAb, PE-Cy5 conjugated [orb3163522]

CD45 Mouse mAb, PE-Cy5 conjugated [orb3163607]

质量保证

常用数据库链接

UniProt 详情

NCBI 基因详情

实验方案信息

可选规格