Avidin/Biotin Blocking Kit Set

SKU: orb2652884

描述

Some tissue sections contain endogenous biotin, biotin binding proteins, lectins or non-specific binding substances. Tissues may also bind avidin, biotin, peroxidase biotin, peroxidase streptavidin in avidin biotin based Immunohistochemistry. These tissues will give high background in the absence of biotinylated secondary antibodies. It may be necessary to block the tissue with avidin, followed by biotin. These reagents are added before the primary antibody.

研究领域

Cell Biology

图片与验证

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应用说明

IHC/ICC procedure for frozen sections, paraffin sections, cell smears. 1. Deparafinize and hydrate tissue sections through xylene or other clearing agents and graded alcohols.(For frozen sections or cell smears; use unfixed, acetone fixed or appropriate fixative for the antigen in question; for cell smears it may be necessary to permealize the cell by detergent, please refer to antibody protocol) 2. Wash 2-3 with distilled or deionized water. 3. Wash slide with Tris/saline buffer, followed by blocking with normal blocking serum, rinse with buffer. 4. Block with reagent A (Avidin) for 5-10 minute, rinse with buffer. 5. Now block with reagent B (Biotin) for 5-10 minutes, wash thoroughly with buffer. Note: If antigen retriever is required it can be applied after this stage. 6. Wash slide with PBS or Tris saline (with 0.02-0.05% nonionic detergent, Triton X100, Tween 20 or NP-40) or washing buffer (Immuno Automation buffer IBSC) 3-5X. 7. Follow instructions for IHC/ICC.

关键属性

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储存与操作

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有效期	12 months from date of receipt.
免责声明	For research use only

相似产品

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Avidin/Biotin Blocking Kit Set [orb2652885]
应用：
ICC, IHC
可选规格： 15 ml

质量保证

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实验方案信息

IHC

免疫组化

查看实验方案

ICC

免疫细胞化学