AVS Bio - hRSPO1-Fc Fusion protein

SKU: R006

特色

描述

RSPO1 is a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeatcontaining G-protein coupled receptors (LGR proteins) and positively regulates the Wnt/β- catenin signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. Although R-spondins are unable to initiate Wnt signaling, they can potently enhance responses to low-dose Wnt proteins.

图片与验证

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应用说明	Human RSPO1-Fc fusion protein has been successfully used in various types of organoids like colon, ileum and duodenum organoids. The working concentration of the product in organoid cultures should be determined experimentally. As a starting concentration 10 – 20 nM can be used. Depending on the type of organoid 0.2 nM WNT surrogate-Fc fusion protein (catalog N001), or similar, can be added.

关键属性

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来源	Recombinant organoid growth factor, Produced via rPEx technology in HEK293E cell line
纯化	Protein A and SEC

储存与操作

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储存	< - 20°C. The product should be stored at - 80°C. After thawing it should be stored in appropriate small aliquots at - 20°C or - 80°C (stable for at least 6 months) or at 4°C for 3 weeks.
形态/外观	solution
缓冲液/防腐剂	PBS, sterile
有效期	For shelf life and expiration details, please refer to the Certificate of Analysis (CoA).
干冰运输	请注意：本产品需要干冰运输，将收取干冰附加费。
免责声明	For research use only

质量保证

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实验方案信息

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