AVS Bio - Human UPAR-(his)N

UPAR is a receptor for urokinase plasminogen activator. It is bound to the plasma membrane via a GPI anchor. However, a soluble protein is also produced in some cell types. It plays a role in localizing and promoting plasmin formation by binding both the proprotein and mature forms of urokinase plasminogen activator (U-PA) and permits the activation of the receptorbound pro-enzyme by plasmin. The protein is subject to negative-feedback regulation by UPA which cleaves it into an inactive form. The mature protein of human UPAR, residues 23 - 335 was over-expressed in HEK293E cells and purified to homogeneity. The protein has a C-terminal pre-peptide, residues 306-335, which are removed in the mature protein, 5 potential N-linked glycosylation sites, and 1 GPIanchor amidated glycine residue. The recombinant protein contains a N-terminal hexahistidine tag. The calculated molecular weight of recombinant human UPAR (excluding glycosylation), including hexahistidine tag, is 36.1 kDa.