Biotin-14-dCTP

SKU: orb533284

描述

Biotin-14-dCTP is enzymatically incorporated into DNA/cDNA as sub-stitute for its natural counterpart dCTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal sub-strate properties for Nick Translation are ensured by a 14-atom linker attached to the N4 position of cytosine. For PCR incorporation experiments e.g. with Taqpolymerase Biotin-11-dCTP or Biotin-16-dCTP are recommended whose Biotin moiety is attached to C5 position of cytidine via a 11-atom or 16-atom linker, respectively. Recommended Biotin-14-dCTP/dCTP ratio for Nick Translation: 50% Biotin-14-dCTP/50% dCTP.

图片与验证

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Item 1 of 1

应用说明	Incorporation into DNA/cDNA by: Nick Translation with DNAse I/DNA Polymerase I & in-house data; Primer Extension with Klenow fragment. Spectroscopic Propertie: λmax 272 nm, ε 13.3 L mmol-1 cm-1 (Tris-HCl, pH 7.5).

关键属性

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MW	Theoretical MW: 905.78 g/mol (free acid); Detected MW: 905.26 g/mol (free acid)
纯度	≥ 95% (HPLC)
分子式	C₃₁H₅₄N₇O₁₆P₃S
SMILES	OP(=O)(O)OP(=O)(O)OP(=O)(O)OC[C@H]1O[C@@H](n2ccc(nc2=O)NCCCCCCNC(=O)CCCCCNC(=O)CCCC[C@@H]2SC[C@@H]3NC(=O)N[C@H]23)C[C@@H]1O

客户验证数据

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以下数据由各地研究人员提交。即使我们尚未自行检测，这些结果也有助于判断该产品是否适合您的应用或种属。

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反应性分布

最新实验

5 Results

稀释

样本

摘要

DNA end-labeling (Micro-C assay)

Drosophila melanogaster

200 μM

Early embryos of Drosophila melanogaster (nuclear cycle 14)

Biotin-14-dCTP is used as part of...

Chromatin conformation capture assay

Drosophila melanogaster, Salmonella enterica

200 μM

Chromatin or genomic DNA derived from fixed cells (e.g., embryos or bacterial cultures)

Biotin-14-dCTP is used as a labeling...

DNA biotinylation

In vitro single-molecule system

33 µM

In vitro assembled transcription elongation complex (EC) on DNA scaffold

Biotin-14-dCTP was used to generate terminally...

Enzymatic DNA end-labeling

In vitro(cell-free)

33 μM

Purified linearized plasmid DNA

Biotin-14-dCTP is used for enzymatic DNA...

Klenow fill-in end-labeling of λ DNA

In vitro(cell-free)

33 μM

Linear double-stranded bacteriophage λ DNA and histones from S. cerevisiae

Biotin-14-dCTP is used for end-labeling DNA...

储存与操作

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储存	store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible.
有效期	12 months from date of receipt.
免责声明	For research use only

别名

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Bio-14-dCTP, Triethylammonium salt

相似产品

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Biotin-14-dCTP [orb1733736]
纯度：
≥ 95% (HPLC)
MW:
Theoretical MW: 905.78 g/mol (free acid); Detected MW: 905.26 g/mol (free acid)
分子式：
C₃₁H₅₄N₇O₁₆P₃S
可选规格： 200 μl

质量保证

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化合物标识

PubChem CID

176417945 ↗

关键属性

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暂无计算属性。

实验方案信息

查看更多实验方案

纯度：	≥ 95% (HPLC)
MW:	Theoretical MW: 905.78 g/mol (free acid); Detected MW: 905.26 g/mol (free acid)
分子式：	C₃₁H₅₄N₇O₁₆P₃S

Biotin-14-dCTP

描述

图片与验证

关键属性

客户验证数据

按应用统计成功数

反应性分布

最新实验

储存与操作

别名

相似产品

Biotin-14-dCTP [orb1733736]

质量保证

化合物标识

关键属性

实验方案信息

可选规格