Cell-mediated Cytotoxicity Assay: Total Cytotoxicity Test

SKU: orb1409923

描述

A single-tube, tri-color fluorescent assay for quantitative assessment of apoptosis and necrosis. CFSE (green) labels target cells, 7-AAD (red) labels dead cells, and SR-FLICA (orange-red) labels active caspases. Analyze by flow cytometry.

图片与验证

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应用说明

1. Prepare samples and controls. 2. Dilute 10X Injection Buffer 1:10 by adding 45 mL diH20. 3. Reconstitute FAM-FLIVO with 50 µL DMSO. 4. Dilute FAM-FLIVO 1:12 with 550 µL 1X Injection Buffer. 5. Inject 100 µL intravenously. 6. Let FAM-FLIVO circulate 30-60 minutes. 7. View live tumor through a window chamber using a fluorescence microscope. 8. If not viewing directly, excise tissue. 9. If desired, label with additional stains, such as Hoechst 33342, or an antibody. 10. If desired, fix cells. 11. Analyze with a fluorescence microscope, flow cytometer, or a window chamber system. FAM-FLIVO excites at 492 nm and emits at 520 nm.

关键属性

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样本类型	Cell culture

储存与操作

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储存	CFSE at ≤ -20°C, other components at 2-8°C
有效期	6 months from date of receipt.
免责声明	For research use only

相似产品

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Cell-mediated Cytotoxicity Assay: Total Cytotoxicity Test [orb1409924]
可选规格： 125 T

质量保证

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实验方案信息

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