CENPA Antibody (APC)

SKU: orb148109

特色

描述

Mouse monoclonal to CENP-A (APC). A replicated chromosome includes two kinetochores that control chromosome segregation during mitosis. The Centromere Protein-A, CENP-A, is a Histone H3-like protein that contains a C-terminal H3-like domain, which is required for centromere localization of CENP-A, and an antigenic N-terminal domain. CENP-A, originally isolated from HeLa cells, is essential for kinetochore targeting of CENP-C. In the presence of DNA CENP-A forms an octa-meric complex with histones H4, H2A, H2B. CENP-A specifically localizes to active centromeres and is a component of specialized centromeric nucleosomes, on which kinetochores are assembled. CENP-A is essential for nucleosomal packaging of centromeric DNA at interphase and functions as a centromere formation marker on the chromosome..

研究领域

Epigenetics & Chromatin

图片与验证

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已测应用	ICC, IF, WB
稀释范围	WB (1:1000), ICC/IF (1:100)
反应性	Human
应用说明	1 µg/ml was sufficient for detection of CENPA in 20 µg of U2OS cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

关键属性

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宿主	Mouse
克隆性	Monoclonal
同种型	IgG1
克隆号	5A7-2E11
免疫原	Synthetic peptide corresponding to a portion of human CENP-A
靶点	CENPA
分子量	18kDa
纯化	Protein G Purified
偶联	APC

储存与操作

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储存	Conjugated antibodies should be stored according to the product label
缓冲液/防腐剂	95.46mM Phosphate, 2.48mM MES and 2mM EDTA
浓度	1 mg/ml
有效期	12 months from date of receipt.
免责声明	For research use only

别名

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CENPA, CENP-A, Centromere autoantigen A, Histone H3-like centromeric protein A

质量保证

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