购物车摘要

购物车里还没有商品。

Ceramide ELISA Kit

SKU: orb1881455
特色特色

描述

Ceramides are key bioactive sphingolipids involved in apoptosis, inflammation, and cellular stress signalling, with established roles in cancer, metabolic disease, and neurodegeneration. Accurate measurement of ceramide levels is essential for studying lipid signalling pathways and disease mechanisms. This Ceramide ELISA kit uses a competitive ELISA assay to enable sensitive and reliable ceramide quantification in serum, plasma, cell lysates, and tissue samples. The assay offers high specificity with minimal cross-reactivity to related lipids, ensuring robust and reproducible results. Ideal for sphingolipid analysis, biomarker research, and drug discovery, this ceramide assay supports both routine laboratory use and high-throughput workflows.

图片与验证

−

关键属性

−
反应性All
样本类型Serum, plasma, cell culture supernatant and other biological samples
检测类型Competitive
检测时间2 hours
范围31.2-2000pg/ml
灵敏度18.75pg/ml

步骤与表现

−
Assay Principle
This kit is based on a competitive ELISA detection method. The microtiter plate is pre-coated with target antigen. During the assay, the target antigen in the sample or standard competes with the immobilized target for binding sites on the biotinylated detection antibody specific to the target antigen. After incubation, excess conjugate and unbound sample or standard are removed by washing. HRP–streptavidin (SABC) is then added to each well and incubated, followed by the addition of TMB substrate solution. The enzymatic reaction is terminated by adding an acidic stop solution, and the color change is measured at 450 nm using a microplate reader. The concentration of the target antigen in the samples is determined by comparison with the standard curve and is inversely proportional to the OD450 value.
Kit Components
1. ELISA Microplate
2. Standards
3. Detection Antibody
4. HRP-Streptavidin Conjugate
5. TMB Substrate
6. Dilution buffers
7. Stop Solution
8. Wash Buffer
9. Plate Sealers
10. Manual
Reagent Preparation
1. Wash Buffer: Prepare the 1X Wash Buffer using distilled water according to the manual.
2. Standard: Perform gradient dilution according to the instructions in the manual.
3. Other Concentrated Reagents: Dilute the concentrated reagents using the Dilution Buffers provided in the kit to 1 X working solutions as instructed in the manual. Always use a clean pipette tip for each different solution.
Assay Procedure
This procedure is for reference only.

1. After the kit equilibrates to room temperature, add standards or samples to each well and incubate.
2. Add biotinylated antibody working solution to each well and incubate.
3. Discard liquid, add wash buffer to each well, wash the plate three times, and blot dry on clean absorbent paper.
4. Add streptavidin-HRP working solution to each well and incubate._x000b_5. Discard liquid, add wash buffer to each well, wash the plate five times, and blot dry on clean absorbent paper.
6. Add TMB substrate solution to each well and incubate in the dark.
7. Add stop solution to each well, mix thoroughly, and immediately read OD at 450 nm.
Materials Required
1. Microplate readers
2. Centrifuge
3. Incubator
4. Automated plate washer
5. Single-channel or multi-channel high-precision pipettes
6. Disposable pipette tips
7. Sterile tubes
8. Eppendorf tubes
9. Absorbent paper
10. Loading slots
Precision
Intra-assay Precision (Precision within an assay): CV% < 8%
Intra-assay precision was evaluated by testing multiple replicates of samples within the same plate.

Inter-assay Precision (precision between assays): CV% < 10%
Inter-assay precision was evaluated by testing samples across different plates.
Calculation of Results
1. Average the duplicate readings for each Standard, Control, and Sample, and subtract the mean optical density of the zero Standard.
2. Construct a standard curve by plotting the target concentration on the y-axis against absorbance on the x-axis and draw a curve through the data points.
3. Determine the sample concentration by substituting the OD450 value into the standard curve. For diluted samples, multiply the calculated value by the corresponding dilution factor.
Curve Fitting Softwares
1. Curve Expert
2. Thermo SkanIt RE
3. SciDAVis
4. LabPlot
5. ……

储存与操作

−
储存2 - 8 °C for 12 months
有效期6 months from date of receipt.
免责声明For research use only

别名

−
Ceramide

相似产品

−
质量保证

质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.

常用数据库链接

基因符号
Ceramide

实验方案信息

查看更多实验方案

Saranya C Reghupaty 1, Laetitia Coassolo 1, Meng Zhao 2, Ramya Lakshmi Narasimhan 3, Aayan Patel 3, Jameel Lone 1, Ewa Bielczyk-Maczynska 4, Niels Banhos Danneskiold-Samsoee 5, Taylor Brown 3, Zewen Jiang 3, Veronica L Li 5, Katrin J Svensson Genetic depletion of adipose-derived Isthmin-1 causes hepatic steatosis Mol Metab, (2025)

PubMed: 40436204

可选规格

请在下方选择规格

48 T
¥ 4,810.00
96 T
¥ 6,370.00
发货Usually dispatched within 5-10 working days
批量询价
需要技术支持？查找经销商退换与税务政策