Chicken Annexin V-Fluorescein Apoptosis Assay Kit

SKU: orb1409911

描述

For quickly and easily distinguishing two populations of dying cells from viable cells using recombinant fluorescein-conjugated Chicken Annexin V and Propidium Iodide. Results can be analyzed via flow cytometer.

研究领域

Cell Biology

图片与验证

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应用说明

1. Prepare samples and controls. 2. Dilute 10X Binding Buffer 1:10 WITH DiH2O. 3. Wash cells twice in ice-cold culture medium or PBS and resuspend in ice-cold 1X Binding Buffer at 5 x 105 – 1 x 106 cells/mL. 4. Reconstitute Equine Annexin V-Fluorescein in 1 mL Annexin Reconstitution Buffer, 1X. 5. Dilute reconstituted Annexin V-Fluorescein 1:5 in PBS. 6. Add diluted Annexin V-Fluorescein to each sample at ~1:10. 7. Dilute PI 1:10 in PBS. 8. Add diluted PI to each sample at ~1:20. 9. Incubate 15 minutes on ice and protected from light. 10. Add 400 µL 1X Binding Buffer to each sample. 11. Analyze with a flow cytometer. Annexin V-Fluorescein excites at 494 nm and emits at 521 nm. PI excites at 536 nm and emits at 617 nm.

关键属性

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样本类型	Cell culture

储存与操作

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储存	2-8°C
有效期	6 months from date of receipt.
免责声明	For research use only

相似产品

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Chicken Annexin V-Fluorescein Apoptosis Assay Kit [orb746785]
可选规格： 500 T

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