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COL3A1 Antibody

SKU: orb345351

描述

Collagen Type III antibody

图片与验证

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已测应用DOT, ELISA, IHC, IP, WB
稀释范围ELISA: 1:5,000 - 1:50,000, IHC: 1:50 - 1:200, IP: 1:100, WB: 1:1,000 - 1:10,000
反应性Bovine, Human, Porcine
应用说明
Anti-Collagen Type III has been tested by dot Blot, western blot, and IHC and is useful for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, native (non-denaturing, non-dissociating) PAGE, immunohistochemistry, and western blotting for highly sensitive qualitative analysis.

关键属性

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抗体类型Primary Antibody
宿主Rabbit
克隆性Polyclonal
同种型IgG
免疫原Collagen Type III from human and bovine placenta
靶点COL3A1
纯度Collagen III Antibody has been prepared by immunoaffinity chromatography using immobilized antigens. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type III collagens and has expected cross-reactivity with Type I and negligible cross reactivity with Type II, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins has not been tested.
偶联Unconjugated

储存与操作

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储存Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
形态/外观Liquid (sterile filtered)
缓冲液/防腐剂Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
浓度1.16 mg/ml
有效期12 months from date of receipt.
干冰运输请注意:本产品需要干冰运输,将收取干冰附加费。
免责声明For research use only

别名

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rabbit anti-Collagen Type III antibody, Collagen type III alpha 1 antibody, Collagen type III alpha antibody, EDS4A antibody, Ehlers Danlos syndrome type IV, autosomal dominant antibody, Fetal collagen antibody, COL3A1, Collagen alpha-1 (III) chain

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质量保证

质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.

UniProt 详情

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暂无 UniProt 数据

NCBI 参考序列

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相关登录号
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实验方案信息

WB
免疫印迹(WB)
查看实验方案
IHC
免疫组化
查看实验方案
ELISA
酶联免疫吸附测定(ELISA)
查看实验方案
IP
免疫沉淀
查看实验方案
DOT
斑点印迹(Dot Blot)
查看实验方案

可选规格

请在下方选择规格

25 μl
免费二抗(20 µl)0/0

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