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描述
研究领域
Cardiovascular Research
图片与验证
−
Item 1 of 12
| 已测应用 | DOT, ELISA, IHC, IP, WB |
|---|---|
| 稀释范围 | ELISA: 1:5,000 - 1:50,000, IHC: 1:50 - 1:200, IP: 1:100, WB: 1:1,000 - 1:10,000 |
| 反应性 | Bovine, Human |
| 应用说明 |
关键属性
−| 抗体类型 | Primary Antibody |
|---|---|
| 宿主 | Rabbit |
| 克隆性 | Polyclonal |
| 同种型 | IgG |
| 免疫原 | Collagen Type IV from human and bovine placenta |
| 靶点 | COL4A1-COL4A6 |
| 纯度 | This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type IV collagens and has negligible cross-reactivity with Type I, II, III, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible. |
| 偶联 | Unconjugated |
储存与操作
−| 储存 | Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. |
|---|---|
| 形态/外观 | Liquid (sterile filtered) |
| 缓冲液/防腐剂 | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| 浓度 | 1.18 mg/mL |
| 有效期 | 12 months from date of receipt. |
| 干冰运输 | 请注意：本产品需要干冰运输，将收取干冰附加费。 |
| 免责声明 | For research use only |
别名
−rabbit anti-Collagen Type IV antibody, Arresten antibody, Canstatin antibody, Collagen Of Basement Membrane Alpha 1 Chain antibody, Collagen alpha-1 (IV) chain, COL4A1
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质量保证
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