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ddATP

SKU: orb65197

描述

ddATP (2',3'-Dideoxyadenosine-5'-triphosphate, Lithium salt) is a high-purity chain-terminating nucleotide analog widely used in DNA sequencing, primer extension, and molecular biology research. As a modified form of dATP, ddATP is enzymatically incorporated into DNA strands but lacks the essential 3'-OH group, resulting in immediate termination of DNA synthesis. This property makes it an essential reagent for Sanger sequencing and controlled DNA polymerization studies.

图片与验证

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应用说明
Spectroscopic Propertie: λmax 259 nm, ε 15.1 L mmol-1 cm-1 (Tris-HCl, pH 7.5).

关键属性

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CAS 号24027-80-3
MWTheoretical MW: 475.18 g/mol (free acid); Detected MW: 475.01 g/mol (free acid)
纯度≥ 98% (HPLC)
分子式C10H16N5O11P3
SMILESOP(=O)(O)OP(=O)(O)OP(=O)(O)OC[C@H]1O[C@@H](n2cnc3c(N)ncnc23)CC1

客户验证数据

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以下数据由各地研究人员提交。即使我们尚未自行检测,这些结果也有助于判断该产品是否适合您的应用或种属。
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反应性分布

最新实验

8 Results
稀释
样本
摘要
Enzymatic nucleotide incorporation assay
Bacteria
500 μM
Purified recombinant proteins (HalB and mutants), nucleotide substrates, and in vitro enzymatic reaction mixtures
DdATP is used in nucleotide synthesis...
Chain-terminating nucleotide for oligo selection
In vitro
2 mM
Immobilized DNA oligos on magnetic beads and PCR-amplified DNA pools
DdATP is used in a synthesis-based...
TdT-mediated DNA labeling for damage detection
Human
30 μM
Designed oligonucleotides with specific DNA damage (e.g., uracil, AP sites) and genomic DNA from cultured cells
DdATP is used in a TdT-mediated...
PH-controlled enzymatic DNA labeling
In vitro system
50 μM
Synthetic single-stranded DNA immobilized on glass surface
DdATP is used in a pH-regulated...
DNA methylation detection (HELMET assay)
Human
20 μM
FFPE tissue sections
DdATP is used in a TdT-mediated...

储存与操作

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储存Store at -20°C. Short term exposure (up to 1 week cumulative) to ambient temperature possible.
有效期12 months from date of receipt.
危害信息Non-Toxic
免责声明For research use only

别名

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2',3'-Dideoxyadenosine-5'-triphosphate, Lithium salt

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    Theoretical MW: ddATP: 475.18 g/mol (free acid), ddCTP: 451.16 g/mol (free acid), ddGTP: 491.18 g/mol (free acid), ddTTP: 466.17 g/mol (free acid)

    ddATP: C 10H16N5O11P3; ddCTP: C 9H16N3O12P3; ddGTP: C 10H16N5O12P3; ddTTP: C 10H17N2O13P3

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    Theoretical MW: 1013.03 g/mol (free acid)

    C37H32Cl2N5O19P3

    120 μl
质量保证

质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.

关键属性

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暂无计算属性。

实验方案信息

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5 x 100 μl
¥ 5,980.00
发货Usually dispatched within 5-10 working days
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