Diaminobezidine (DAB) Kit

SKU: orb2652870

描述

Peroxidase chromogen-substrate 3-3´ diaminobezidine (DAB) reagent is an excellent chromogen system that is used in Immunohistochemistry (IHC) and Immunocytochemistry (ICC).

图片与验证

−

应用说明

To prepare the reagent, add two drops of Chromogen reagent to one milliliter of Buffer Substrate in a test tube and mix well. This ready-to-use reagent remains stable for several hours, and any unused DAB solution should be discarded according to local regulations. For IHC and ICC applications, following the peroxidase reaction, wash the slide with buffer and distilled or deionized water. Apply a few drops of the ready-to-use DAB reagent to the tissue slides for 5 to 10 minutes at room temperature. Wash two to five times with buffer, followed by a rinse with distilled or deionized water. Apply a counterstain compatible with DAB, such as hematoxylin, for 30 to 60 seconds. Wash with tap water or a buffer with a pH of 7.4 or higher, followed by a rinse with distilled or deionized water. Finally, mount the slide with aqueous or organic mounting medium after dehydration as specified in the mounting protocol.

关键属性

−

储存与操作

−

储存	2-8 °C
有效期	12 months from date of receipt.
免责声明	For research use only

相似产品

−

Diaminobezidine (DAB) Kit [orb2652871]
应用：
ICC, IHC
可选规格： 200 ml
Diaminobezidine (DAB) Kit [orb2652872]
应用：
ICC, IHC
可选规格： 100 ml

质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.

实验方案信息

IHC

免疫组化

查看实验方案

ICC

免疫细胞化学

查看实验方案

查看更多实验方案