Donkey anti Goat IgG (heavy and light chains) Antibody (Biotin)

SKU: orb22021

描述

Biotin-conjugated IgG fraction of polyclonal donkey antiserum to goat IgG, heavy and light chains. To identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immuno- fluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of goat origin in the middle layer of the indirect test procedure. As an extra step an avidin or streptavidin of the user's choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions fir histochemical and cytochemical use are usually between 1:100 and 1:500; in ELISA and comparable no-precipitating antibody-binding assays between 1: 1,000 and 1:10,000.

研究领域

Immunology & Inflammation; Cell Biology

图片与验证

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反应性	Goat

关键属性

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抗体类型	Secondary Antibody
宿主	Donkey
克隆性	Polyclonal
偶联	Biotin

储存与操作

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储存	The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
形态/外观	Biotin-coupled purified hyperimmune donkey IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added.
有效期	12 months from date of receipt.
免责声明	For research use only

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实验方案信息

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