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dsDNA Antibody

SKU: orb749982
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描述

This mAb recognizes the double stranded DNA in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in human cells. This mAb produces a homogeneous staining pattern in the nucleus of normal and malignant cells. Double Stranded deoxyribonucleic acid (ds DNA) is the genetic material of all cells and many viruses and is a polymer of nucleotides. The monomer consists of phosphorylated 2-deoxyribose N-glycosidically linked to one of four bases, adenine, cytosine, guanine or thymine. These are linked together by 3',5'-phosphodiester bridges. In the Watson-Crick double-helix model, two complementary strands are wound in a right-handed helix and held together by hydrogen bonds between complementary base pairs.

图片与验证

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已测应用FACS, IHC-P
稀释范围Flow Cytometry: 1-2ug/million cells in 0.1ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1)
反应性Human
应用说明
Optimal dilution of the dsDNA antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

关键属性

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抗体类型Primary Antibody
宿主Mouse
克隆性Monoclonal
同种型Mouse IgG3, kappa
克隆号AE-2
免疫原Nuclei of Burkitt's cells were used as the immunogen for the dsDNA antibody.
纯化Protein G affinity chromatography
偶联Unconjugated

储存与操作

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储存Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
缓冲液/防腐剂0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
有效期12 months from date of receipt.
免责声明For research use only

相似产品

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质量保证

质量保证

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常用数据库链接

UniProt
Not Known

UniProt 详情

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暂无 UniProt 数据

实验方案信息

IHC-P
石蜡切片免疫组化
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FACS
荧光激活细胞分选（FACS，流式细胞术）
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可选规格

请在下方选择规格

20 μg
¥ 4,810.00
100 μg
¥ 9,750.00
选择偶联或无载体版本
免费二抗（20 µl）0/0

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发货Usually dispatched within 5-10 working days
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