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DYKDDDDK Tag (FLAG) Antibody Peroxidase Conjugated

SKU: orb344540

描述

DYKDDDDK antibody (Peroxidase)

图片与验证

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已测应用ELISA, IHC, WB
稀释范围ELISA: 1:10,000-1:50,000, IHC: 1:500-1:2,500, WB: 1:10,000
应用说明
Anti-FLAG Peroxidase has been tested by ELISA and western blot. This antibody is optimally suited for monitoring the expression of FLAG tagged fusion proteins. As such, this antibody can be used to identify fusion proteins containing the FLAG epitope. The antibody recognizes the epitope tag fused to either the amino- or carboxy- termini of targeted proteins. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag.

关键属性

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抗体类型Primary Antibody
宿主Mouse
克隆性Monoclonal
同种型IgG2a
克隆号29E4.G7
免疫原This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide.
纯度This antibody is directed against the FLAG epitope tag and is useful in determining its presence in over expressed proteins in various assays. The antibody recognizes the FLAG epitope tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
偶联HRP

储存与操作

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储存Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
形态/外观Lyophilized
缓冲液/防腐剂Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
浓度1.0 mg/mL
有效期12 months from date of receipt.
免责声明For research use only

别名

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mouse anti-FLAG tag HRP conjugation, Enterokinase Cleavage Site (ECS), peroxidase conjugated mouse anti-DYKDDDDK, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys

相似产品

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质量保证

质量保证

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实验方案信息

WB
免疫印迹(WB)
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IHC
免疫组化
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ELISA
酶联免疫吸附测定(ELISA)
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可选规格

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100 μg
¥ 8,840.00
选择偶联或无载体版本
免费二抗(20 µl)0/0

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发货Usually dispatched within 5-10 working days
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