DYKDDDDK Tag (FLAG) Antibody Agarose Conjugated

SKU: orb344560

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描述

Mouse monoclonal antibody against DYKDDDDK (FLAG tag) conjugated to Agarose.

研究领域

Molecular Biology

图片与验证

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Item 1 of 3

已测应用	IP, WB
稀释范围	IP: 10 µL resin binds >1 µg FLAG fusion protein
应用说明	Anti-DYKDDDDK Affinity Gel has been tested by SDS-Page, IP, and western blot and is optimally suited for immunoprecipitation and purification of FLAG tagged fusion proteins. Anti-DYKDDDDK Affinity Gel antibody recognizes the FLAG epitope tag fused to either the amino- or carboxy- terminal ends or an internal location of targeted fusion proteins. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag. Use D-Y-K-D-D-D-D-K peptide for competitive elution to recover fusion protein (see protocol). Refer to the Protocol for complete instructions for use including preferred buffers for elution. Do not use buffers that may denature the anti-DYKDDDDK antibody.

关键属性

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抗体类型	Primary Antibody
宿主	Mouse
克隆性	Monoclonal
同种型	IgG2a
克隆号	29E4.G7
免疫原	Anti-DYKDDDDK Affinity Gel antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH.
纯度	Anti-DYKDDDDK Affinity Gel is a purified mouse IgG2a monoclonal antibody coupled to activated agarose. This product is intended for purification of proteins containing the FLAG epitope tag sequence. Binding Specificity: Anti-DYKDDDDK Affinity Gel binds the FLAG epitope tag sequence (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) fused to the amino terminal, carboxy terminal or internal locations of targeted recombinant proteins expressed in transfected or transformed cells. D-Y-K-D-D-D-D-K peptide is recommended for competitive elution to recover fusion protein (see protocol).
偶联	Agarose

储存与操作

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储存	Store vial at 4°C prior to opening.
形态/外观	Suspension of agarose beads
缓冲液/防腐剂	Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
浓度	0.99 mg
有效期	12 months from date of receipt.
免责声明	For research use only

别名

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mouse anti-FLAG tag agarose gel, mouse anti-DYKDDDDK agarose gel, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, D-Y-K-D-D-D-D-K, DDK, FLAG, FLAG antibody, anti-Flag, anti-DDK, DDK antibody

相似产品

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Agarose Conjugated DDDDK Tag Mouse Monoclonal Antibody [orb1173291]
应用：
IP
克隆性：
Monoclonal
偶联：
Unconjugated
可选规格： 1 ml, 5 ml

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实验方案信息

免疫印迹（WB）

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