Recombinant EpCAM Antibody / Rabbit Monoclonal

SKU: orb750182

描述

Recognizes a 40-43kDa transmembrane epithelial glycoprotein, identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). EpCAM is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. It is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. This epithelial antigen plays an important role as a tumor-cell marker in lymph nodes from patients with esophageal carcinoma otherwise classified as node-negative. Epithelial antigen has also been suggested as a discriminator between basal cell and baso-squamous carcinomas, and squamous cell carcinoma of the skin.

研究领域

Immunology & Inflammation

图片与验证

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已测应用	FACS, IF, IHC-P
稀释范围	Flow cytometry: 1-2ug/10^6 cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Immunofluorescence: 1-2ug/ml
反应性	Human
应用说明	Titering of the recombinant EpCAM antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

关键属性

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抗体类型	Primary Antibody
宿主	Rabbit
克隆性	Recombinant
同种型	Rabbit IgG, kappa
克隆号	EGP40/1555R
免疫原	Recombinant full length protein was used as the immunogen for the recombinant EpCAM antibody.
纯化	Protein A affinity chromatography
偶联	Unconjugated

储存与操作

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储存	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
缓冲液/防腐剂	0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
有效期	12 months from date of receipt.
免责声明	For research use only

相似产品

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Item 1 of 6
Recombinant EpCAM Antibody / Rabbit Monoclonal [orb2642100]
应用：
FACS, IF, IHC-P
反应性：
Human
种属/宿主：
Rabbit
克隆性：
Recombinant
偶联：
Unconjugated
可选规格： 100 μg
Item 1 of 3
Recombinant EpCAM Antibody / Rabbit Monoclonal [orb2642099]
应用：
IHC-P
反应性：
Human
种属/宿主：
Rabbit
克隆性：
Recombinant
偶联：
Unconjugated
可选规格： 7 ml

质量保证

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