F(ab')2 Human IgG IgA IgM (H&L) Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed

SKU: orb348170

该产品可以定制

描述

F(ab')2 Human IgG IgA IgM (H&L) antibody (Alkaline Phosphatase)

研究领域

Signal Transduction

图片与验证

−

已测应用	ELISA, IHC, WB
稀释范围	ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
反应性	Human
应用说明	F(ab')2 Anti-Human IgG IgA IgM (H&L) Alkaline Phosphate Antibody has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product. Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

关键属性

−

抗体类型	Secondary Antibody
宿主	Goat
克隆性	Polyclonal
同种型	IgG F(ab')2
免疫原	Human IgG, IgA, and IgM whole molecules
纯度	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase and anti-Goat Serum. This reagent is suitable for the detection of all human immunoglobulins and chain combinations. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Mouse Serum Proteins.
偶联	AP

储存与操作

−

储存	Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
形态/外观	Liquid (sterile filtered)
缓冲液/防腐剂	Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
浓度	0.5 mg/mL
有效期	12 months from date of receipt.
免责声明	For research use only

别名

−

Goat F(ab')2 Anti-Human IgG IgA IgM Alkaline phosphatase Conjugated Antibody Pre-Adsorbed, Goat Fab2 Anti-Human IgGAM Antibody alk phos Conjugation

质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.