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F(ab')2 Mouse IgG (H&L) Antibody Biotin Conjugated Pre-Adsorbed

SKU: orb348279

描述

F(ab')2 Mouse IgG (H&L) antibody (Biotin)

图片与验证

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已测应用ELISA, IHC, WB
稀释范围ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
反应性Mouse
应用说明
F(ab')2 Anti-Mouse IgG (H&L) Biotin has been tested by ELISA and is assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code as a substrate for 30 minutes at room temperature. A working dilution of 1:7,000 to 1:70,000 of the reconstitution concentration is suggested for this product. Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. Reconstitution Buffer: Restore with deionized water (or equivalent). Reconstitution Volume: 500 µL

关键属性

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抗体类型Secondary Antibody
宿主Rabbit
克隆性Polyclonal
同种型IgG F(ab')2
免疫原Mouse IgG whole molecule
纯度This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Human Serum Proteins.
偶联Biotin

储存与操作

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储存Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
形态/外观Lyophilized
缓冲液/防腐剂Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
浓度0.5 mg/mL
有效期12 months from date of receipt.
免责声明For research use only

别名

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Rabbit F(ab')2 Anti-Mouse IgG Antibody Biotin Conjugation, Rabbit Fab2 Anti-Mouse IgG Biotin Conjugated Antibody

相似产品

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质量保证

质量保证

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实验方案信息

WB
免疫印迹(WB)
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IHC
免疫组化
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ELISA
酶联免疫吸附测定(ELISA)
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可选规格

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500 μg
¥ 6,240.00
免费二抗(20 µl)0/0

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发货Usually dispatched within 5-10 working days
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