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F(ab')2 Mouse IgG (H&L) Antibody Phycoerythrin Conjugated Pre-Adsorbed

SKU: orb348288

描述

F(ab')2 MOUSE IgG (H&L) antibody (RPE)

图片与验证

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已测应用DOT, FC, IF
稀释范围FC: 1:100 - 1:250, IF: 1:100 - 1:250
反应性Mouse
应用说明
F(ab')2 Anti-Mouse IgG Phycoerythrin has been tested by dot blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.

关键属性

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抗体类型Secondary Antibody
宿主Donkey
克隆性Polyclonal
同种型IgG F(ab')2
免疫原F(ab')2 Anti-Mouse IgG whole molecule was produced by repeated immunization with Mouse IgG whole molecule in donkey.
纯度F(ab')2 fragment PE conjugated secondary antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Donkey Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c), Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat or Sheep Serum Proteins.
偶联RPE

储存与操作

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储存Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
形态/外观Lyophilized
缓冲液/防腐剂Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
浓度0.5 mg/mL
有效期12 months from date of receipt.
免责声明For research use only

别名

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Donkey F(ab')2 Anti-Mouse IgG Antibody Phycoerythrin Conjugation, Donkey Fab2 Anti-Mouse IgG PE Conjugated Antibody

相似产品

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质量保证

质量保证

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实验方案信息

FC
流式细胞术
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IF
免疫荧光
查看实验方案
DOT
斑点印迹(Dot Blot)
查看实验方案

可选规格

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500 μg
¥ 9,620.00
免费二抗(20 µl)0/0

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发货Usually dispatched within 5-10 working days
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