F(ab')2 Rat IgG (H&L) Antibody Phycoerythrin Conjugated Pre-Adsorbed

SKU: orb348368

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描述

F(ab')2 Rat IgG (H&L) antibody (RPE)

图片与验证

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反应性	Rat
应用说明	Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.

反应性

Rat

应用说明

Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.

关键属性

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抗体类型	Secondary Antibody
宿主	Rabbit
克隆性	Polyclonal
同种型	IgG F(ab')2
免疫原	Rat IgG whole molecule
纯度	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Coupling to R-PE was followed by size exclusion chromatography to purify conjugate from unreacted R-PE and antibody. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Rabbit Serum, Rat IgG and Rat Serum. No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Bovine, Horse, Human, Mouse, Rabbit & Sheep Serum Proteins.
偶联	RPE

储存与操作

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储存	Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
形态/外观	Lyophilized
缓冲液/防腐剂	Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
浓度	0.5 mg/mL
有效期	12 months from date of receipt.
免责声明	For research use only

别名

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Rabbit F(ab')2 Anti-Rat IgG Phycoerythrin Conjugated Pre-Adsorbed Antibody, Rabbit Fab2 Anti-Rat IgG Antibody PE Conjugation

相似产品

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F(ab')2 Rat IgG (H&L) Antibody Phycoerythrin Conjugated Pre-Adsorbed [orb348351]
应用：
DOT, IF, WB
反应性：
Rat
种属/宿主：
Goat
克隆性：
Polyclonal
偶联：
RPE
可选规格： 500 μg
F(ab')2 Rat IgG (H&L) Antibody Phycoerythrin Conjugated Pre-Adsorbed [orb348376]
应用：
FC, IF
反应性：
Rat
种属/宿主：
Donkey
克隆性：
Polyclonal
偶联：
RPE
可选规格： 500 μg

质量保证

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实验方案信息

查看更多实验方案

应用：	DOT, IF, WB
反应性：	Rat
种属/宿主：	Goat
克隆性：	Polyclonal
偶联：	RPE

应用：	FC, IF
反应性：	Rat
种属/宿主：	Donkey
克隆性：	Polyclonal
偶联：	RPE