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FAM-FLICA Poly Caspase Assay Kit

SKU: orb1409971

描述

This FLICA (Fluorescent Labeled Inhibitors of CAspases) kit is designed to detect active caspase enzymes. This in vitro assay employs the fluorescent inhibitor probe FAM-VAD-FMK to label active caspase enzymes in living cells. Samples can be analyzed using fluorescence microscopy, a fluorescence plate reader, or by flow cytometry. Our Green Fluorescent Poly (active) Caspase FAM FLICA Inhibitor Reagent (FAM-VAD-FMK) can be used as a general reagent to detect apoptosis as it is recognized by many types of activated caspases. The probe measures the intracellular process of apoptosis instead of a side-effect, such as the turnover of phosphatidyl serine, and eliminates the incidence of false positives that often plagues methods like Annexin V and TUNEL staining.

研究领域

Cell Biology

图片与验证

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应用说明
1. Prepare samples and controls. 2. Dilute 10X Apoptosis Wash Buffer 1:10 with diH20. 3. Reconstitute FLICA with 50 µL DMSO. 4. Dilute FLICA 1:5 by adding 200 µL PBS. 5. Add diluted FLICA to each sample at 1:30 (e.g. add 10 µL to 290 µL of cultured cells). 6. Incubate approximately 1 hour. 7. Remove media and wash cells 3 times: add 1X Apoptosis Wash Buffer and spin cells. 8. If desired, label with additional stains, such as Hoechst, Propidium Iodide, 7-AAD, or an antibody. 9. If desired, fix cells. 10. Analyze with a fluorescence microscope, fluorescence plate reader, or flow cytometer. This product excites at 492 nm and emits at 520 nm. If working with adherent cells, please see the manual for additional protocols.

关键属性

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样本类型Cell culture

储存与操作

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储存2-8°C
有效期6 months from date of receipt.
免责声明For research use only

相似产品

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质量保证

质量保证

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实验方案信息

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100 T
¥ 12,870.00
发货Usually dispatched within 5-10 working days
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