FLICA 660 Caspase-1 Assay Kit

SKU: orb1409917

描述

This in vitro assay employs the far-red fluorescent inhibitor probe 660-YVAD-FMK to label active caspase-1 enzyme in living cells. Analyze samples using fluorescence microscopy, or flow cytometry.

研究领域

Cell Biology

图片与验证

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应用说明

1. Prepare samples and controls. 2. Dilute 10X Binding Buffer 1:10 with diH2O. 3. Wash cells in ice-cold culture medium or PBS and resuspend in ice-cold 1X Binding Buffer. 4. Dilute Annexin V-FITC 1:10 in PBS. 5. Dilute Propidium Iodide (PI) 1:2 in PBS. 6. Add diluted Annexin V-FITC to each sample at a ratio of 1:20 (5 µL per 100 µL aliquot of cultured cells). 7. Add diluted PI to each sample at a ratio of 1:20 (5 µL per 100 µL aliquot of cultured cells). 8. Incubate ~10 minutes, protected from light. 9. Dilute samples by adding 250 µL 1X Binding Buffer to each. 10. Analyze with a flow cytometer. Annexin V-FITC optimally excites at 494 nm and emits at 519 nm (typically read in FL1). PI optimally excites at 536 nm and has a peak emission at 617 nm (typically read in FL3).

关键属性

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样本类型	Cell culture

储存与操作

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储存	2-8°C
有效期	6 months from date of receipt.
免责声明	For research use only

相似产品

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FLICA 660 Caspase 1 Assay Kit [orb322262]
可选规格： 25 - 50 T

质量保证

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实验方案信息

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