FLICA 660 Caspase 3/7 Assay Kit

SKU: orb1409915

描述

This in vitro assay employs the far-red fluorescent inhibitor probe 660-DEVD-FMK to label active caspase 3 and caspase 7 enzymes in living cells. Analyze the fluorescent signal using fluorescence microscopy or flow cytometry.

研究领域

Cell Biology

图片与验证

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应用说明

1. Prepare Assay buffer; dilute Assay Buffer Concentrate 1:5 in diH2O. 2. Prepare samples; dilute > 1:10 in Assay Buffer. 3. Prepare standard curve and zero (blank) standard. 4. Add 50 µL blanks, standards, and samples to plate. 5. Add 25 µL Fluorescent H2O2 Detection Substrate to plate. 6. Prepare HRP; dilute Horseradish Peroxidase Concentrate 1:100 in Assay Buffer. 7. Add 25 µL HRP Preparation to plate. 8. Incubate 15 minutes at room temperature. 9. Read fluorescence with excitation at 570 nm and emission at 590 nm.

关键属性

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样本类型	Cell culture

储存与操作

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储存	2-8°C
有效期	6 months from date of receipt.
免责声明	For research use only

相似产品

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FLICA 660 Caspase 3/7 Assay Kit [orb322263]
可选规格： 25 - 50 T

质量保证

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实验方案信息

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