Recombinant GFAP Antibody / Rabbit Monoclonal

SKU: orb606415

描述

This mAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

研究领域

Neuroscience

图片与验证

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已测应用	FACS, IF, IHC-P, WB
稀释范围	Flow cytometry: 1-2ug/10^6 cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Immunofluorescence: 1-2ug/ml,Western blot: 2-4ug/ml
反应性	Human
应用说明	The optimal dilution of the recombinant GFAP antibody for each application should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

关键属性

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抗体类型	Primary Antibody
宿主	Rabbit
克隆性	Recombinant
同种型	Rabbit IgG, kappa
克隆号	ASTRO/1974R
免疫原	GFAP isolated from pig spinal cord was used as the immunogen for this recombinant GFAP antibody.
纯化	Protein A affinity chromatography
偶联	Unconjugated

储存与操作

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储存	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
缓冲液/防腐剂	0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
有效期	12 months from date of receipt.
免责声明	For research use only

相似产品

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Item 1 of 4
Recombinant GFAP Antibody / Rabbit Monoclonal [orb2640615]
应用：
FACS, IF, IHC-P, WB
反应性：
Human
种属/宿主：
Rabbit
克隆性：
Recombinant
偶联：
Unconjugated
可选规格： 100 μg
Item 1 of 2
Recombinant GFAP Antibody / Rabbit Monoclonal [orb2640614]
应用：
IHC-P
反应性：
Human
种属/宿主：
Rabbit
克隆性：
Recombinant
偶联：
Unconjugated
可选规格： 7 ml

质量保证

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