GFP Antibody

SKU: orb345288

描述

GFP antibody

图片与验证

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Item 1 of 28

已测应用	ELISA, IF, IHC, WB
稀释范围	ELISA: 1:10,000 - 1:30,000, IHC: 1:200 - 1:1,000, IF: 1:500, WB: 1:1,000 - 1:10,000
反应性	Other
应用说明	Anti-GFP is designed to detect GFP and its variants. Goat Anti-GFP has been tested by ELISA, SDS-PAGE, Western blot, and Immunofluorescence. This product is ideal for western blotting, ELISA, immunofluorescence, IHC, and IP. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with Biorbyt's monoclonal anti-GFP using either form of the antibody as the capture or detection antibody. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin-HRP . Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and detects GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP-containing proteins on western blots. Researchers should determine optimal titers for applications.

关键属性

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抗体类型	Primary Antibody
宿主	Goat
克隆性	Polyclonal
同种型	IgG
免疫原	The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
纯度	GFP antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria). No reaction was observed against Human, Mouse or Rat serum proteins.
偶联	Unconjugated

储存与操作

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储存	Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
形态/外观	Liquid (sterile filtered)
缓冲液/防腐剂	Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
浓度	1.18 mg/mL
有效期	12 months from date of receipt.
干冰运输	请注意：本产品需要干冰运输，将收取干冰附加费。
免责声明	For research use only

别名

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goat anti-GFP antibody, GFP, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish

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反应性：
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ELISA, FC, IF, IP, WB
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质量保证

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