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GFP Control protein

SKU: orb345957

描述

GFP Control protein

图片与验证

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已测应用SDS-PAGE, WB
应用说明
GFP Control is suitable as a control for polyclonal or monoclonal Anti-GFP in immunological assays. Researchers should determine optimal titers for applications. The molecular weight of GFP is 28 kDa. Green Fluorescent Protein has been tested in SDS-page, western blot, and immunoprecipitation.

关键属性

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表达系统E. coli
偶联Unconjugated
纯度GFP control protein was synthesized with an amino terminal 6XHIS tag and was expressed in E. coli and purified by sequential hydrophobic interaction chromatography, DEAE chromatography and size-exclusion chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-GFP. Analysis by SDS-PAGE resulted in a pattern consistent with purified GFP and was estimated to be greater than 90% pure.

储存与操作

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储存Store GFP at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.
形态/外观Liquid (sterile filtered)
缓冲液/防腐剂Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
浓度1.0 mg/mL
有效期6 months from date of receipt.
干冰运输请注意:本产品需要干冰运输,将收取干冰附加费。
免责声明For research use only

别名

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control protein, rGFP protein, GFP fusion protein, GFP control

相似产品

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质量保证

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UniProt 详情

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暂无 UniProt 数据

实验方案信息

蛋白处理与保存指南
保存指南
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WB
免疫印迹(WB)
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SDS-PAGE
十二烷基硫酸钠聚丙烯酰胺凝胶电泳(SDS-PAGE)
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可选规格

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100 μg