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CARD11 Antibody

SKU: orb18487

描述

Goat polyclonal antibody to CARD11

研究领域

Signal Transduction

图片与验证

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已测应用ELISA, IHC, WB
稀释范围ELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 2-5 μg/ml
反应性Human
预测反应性Bovine, Canine, Mouse
应用说明
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: Immunohistochemistry: In paraffin embedded Human Spleen shows nuclear staining. Recommended concentration, 2-5ug/ml.WB: Approx 150+65kDa bands observed in lysates of cell line Jurkat (calculated MW of 133kDa according to NP_115791.2). A similar pattern was observed in HEK293 transiently expressing CARD11 (pciture not shown). These bands are not observed in the non-transfected HEK293. Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

关键属性

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克隆性Polyclonal
靶点CARD11
蛋白序列QRKTIWVDEDQL
分子量133
纯化Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
偶联Unconjugated

储存与操作

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储存Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
缓冲液/防腐剂Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
有效期12 months from date of receipt.
免责声明For research use only

别名

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anti CARD11 antibody, member 11 antibody, anti BIMP3 antibody, anti CARMA1 antibody, anti MGC133069 antibody, anti card-maguk protein 1 antibody, anti bcl10-interacting maguk protein 3 antibody

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质量保证

质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.

NCBI 基因详情

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暂无 NCBI 基因数据

实验方案信息

WB
免疫印迹(WB)
查看实验方案
IHC
免疫组化
查看实验方案
ELISA
酶联免疫吸附测定(ELISA)
查看实验方案

可选规格

请在下方选择规格

100 μg
¥ 7,280.00
选择偶联或无载体版本
免费二抗(20 µl)0/0

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发货Usually dispatched within 5-10 working days
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