SEPT6 Goat Polyclonal Antibody

SKU: orb20255

描述

This product is a goat polyclonal antibody targeting SEPT6, supplied unconjugated at 0.5 mg/ml. Validated for multiple applications including ELISA, flow cytometry, immunofluorescence, immunohistochemistry, and Western blot, it exhibits reactivity with human samples and shows predicted reactivity with bovine and canine species.

研究领域

Stem Cell & Developmental Biology; Cell Biology

图片与验证

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Item 1 of 6

已测应用	ELISA, FC, IF, IHC, WB
稀释范围	Peptide ELISA: antibody detection limit dilution 1:4000. Western blot: Approx 50kDa band observed in Human Testis, Duodenum, Kidney and Tonsil lysates, and in lysates of cell lines Daudi, Jurkat and MOLT-4 (calculated MW of 49.7kDa according to NP_055944.2). Recommended concentration: 0.1-1µg/ml. Primary incubation 1 hour at room temperature. IHC: In paraffin embedded Human Tonsil shows specific staining in dividing cells just outside a germinal centre. Recommended concentration: 3-5µg/ml. Immunofluorescence: Strong expression of the protein seen in A431 and U2OS cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.
反应性	Human
预测反应性	Bovine, Canine

关键属性

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克隆性	Polyclonal
免疫原	Peptide with sequence C-DEVNAFKQRKTA, from the internal region of the protein sequence according to NP_665798.1; NP_055944.2; NP_665801.1.
靶点	SEPT6
蛋白序列	DEVNAFKQRKTA
分子量	48.9 kDa; 49.7 kDa; 49.2 kDa
纯化	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
偶联	Unconjugated

储存与操作

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储存	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
缓冲液/防腐剂	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
有效期	12 months from date of receipt.
免责声明	For research use only

别名

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anti SEPT6 antibody, anti septin 6 antibody, anti KIAA0128 antibody, anti MGC16619 antibody, anti MGC20339 antibody, anti SEP2 antibody, anti SEPT2 antibody, anti septin 2 antibody

相似产品

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Item 1 of 1
SEPT6, Biotinylated Antibody [orb403012]
应用：
ELISA, WB
预测反应性：
Bovine, Canine
反应性：
Human
克隆性：
Polyclonal
偶联：
Unconjugated
可选规格： 100 μg

质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.