Goat anti Swine IgM (Fc specific) Antibody (HRP)

SKU: orb21716

描述

Horseradish peroxidase-conjugated IgG fraction of polyclonal goat antiserum to swine IgM, Fc specific. In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of swine origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in swine serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:50 and 1:250; in ELISA and comparable non-precipitating antibody-binding assays between 1:1,000 and 1:5,000.

研究领域

Cell Biology

图片与验证

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已测应用	DOT, ELISA, ICC, IHC-P, WB
反应性	Porcine

关键属性

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抗体类型	Secondary Antibody
宿主	Goat
克隆性	Polyclonal
偶联	HRP

储存与操作

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储存	The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
浓度	10 mg/ml total IgG after reconstitution in 1 ml sterile distilled water.
有效期	12 months from date of receipt.
免责声明	For research use only

质量保证

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