Goat IgG anti Hamster IgG (heavy and light chains) Antibody (HRP)

SKU: orb22039

描述

Horseradish peroxidase-conjugated IgG fraction of polyclonal goat antiserum to hamster IgG, heavy and light chains. In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG, antigen or antibody, of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure a specific IgG in hamster serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electron-dense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1: 500; in ELISA and comparable non-precipitating antibody-binding assays between 1:1,000 and 1:5,000.

研究领域

Immunology & Inflammation; Cell Biology

图片与验证

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已测应用	DOT, ELISA, ICC, WB
反应性	Hamster

关键属性

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抗体类型	Secondary Antibody
宿主	Goat
克隆性	Polyclonal
偶联	HRP

储存与操作

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储存	The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
形态/外观	Peroxidase-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
有效期	12 months from date of receipt.
免责声明	For research use only

质量保证

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