GW 4064

SKU: orb1305997

描述

GW 4064 is a potent and selective synthetic agonist of the farnesoid X receptor (FXR), exhibiting an EC50 of 65 nM. This tool compound is widely used in both in vitro and in vivo research to investigate FXR's role in bile acid homeostasis, metabolic disorders, and liver diseases.

研究领域

Cell Biology, Metabolism Research

图片与验证

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关键属性

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CAS 号	278779-30-9
MW	542.84
纯度	99.94% (May vary between batches)
分子式	C₂₈H₂₂Cl₃NO₄
SMILES	CC(C)c1onc(c1COc1ccc(C=Cc2cccc(c2)C(O)=O)c(Cl)c1)-c1c(Cl)cccc1Cl
靶点	FXR,Autophagy
溶解性	DMSO:50 mg/mL (92.11 mM);Ethanol:5.4 mg/mL (9.95 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (3.68 mM)

生物活性

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靶点 IC50	FXR:65 nM(EC50)
体内	In CV-1 cells transfected with mouse FXR expression vectors (EC50=80 nM) and in CV-1 cells transfected with human FXR expression vectors (EC50=90 nM), GW 4064 functions as a selective non-steroidal FXR agonist, effectively exerting agonistic effects.
体外	In Fisher rats, oral administration of GW 4064 (ED50 = 20 mg/kg) effectively reduces serum triglycerides.
细胞研究	GW 4064 is dissolved in DMSO and stored, and then diluted with appropriate media before use. Mouse liver cells (BNL CL.2) are maintained in a humidified incubator under 5% CO2 at 37°C in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% Penicillin/Streptomycin. When cells are divided into six-well plates and reach ~90% confluence, sub-confluent cells are washed three times with phosphate buffered saline (PBS) and replaced with serum-free DMEM supplemented with 1% fatty acid-free BSA. Oleic acid (final concentration 500 μM) and GW4064 at various concentrations are added and incubated for 24 h. Cells are then fixed with 4% formaldehyde for Oil Red O staining or harvested for protein and western blot analysis.

储存与操作

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储存	-20°C
有效期	12 months from date of receipt.
免责声明	For research use only

别名

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Inhibitor, NR1H4, inhibit, GW 4064, GW4064, GW-4064, FXR, Autophagy

相似产品

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dual FXR/PPARδ agonist-2 [orb3173250]
可选规格： 50 mg, 10 mg
GW-4064 [orb1225443]
纯度：
>98% (HPLC)
CAS 号：
278779-30-9
MW:
542.8376
分子式：
C₂₈H₂₂Cl₃NO₄
可选规格： 5 mg, 10 mg, 100 mg, 500 mg, 1 g, 200 mg, 25 mg, 50 mg

质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.

化合物标识

PubChem CID

44483281 ↗

关键属性

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暂无计算属性。

实验方案信息

查看更多实验方案

纯度：	>98% (HPLC)
CAS 号：	278779-30-9
MW:	542.8376
分子式：	C₂₈H₂₂Cl₃NO₄

GW 4064

描述

研究领域

图片与验证

关键属性

生物活性

储存与操作

别名

相似产品

dual FXR/PPARδ agonist-2 [orb3173250]

GW-4064 [orb1225443]

质量保证

化合物标识

关键属性

实验方案信息

可选规格