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HA Epitope Tag Antibody

SKU: orb345398

描述

HA EPITOPE TAG antibody

图片与验证

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已测应用ELISA, IHC, WB
稀释范围ELISA: 1:10,000 - 1:100,000, IHC: 1:500 - 1:2,000, WB: 1:2,000 - 1:10,000
应用说明
Anti-HA is optimally suited for monitoring the expression of HA-tagged fusion proteins. As such, anti-HA/HA can be used to identify fusion proteins containing the HA epitope. The antibody recognizes the HA epitope tag fused to the amino- or carboxy- termini of targeted proteins, as expressed in many commonly used expression vectors. This antibody has been tested by ELISA, immunohistochemistry, and western blotting against both the immunizing peptide and HA containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation, immunocytochemistry, and other immunodetection techniques. Affinity purification of the polyclonal antibody results in very low background levels in assays and low cross-reactivity with other cellular proteins.

关键属性

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抗体类型Primary Antibody
宿主Rabbit
克隆性Polyclonal
同种型IgG
免疫原Anti-HA antibody was purified from whole rabbit serum prepared by repeated immunizations with the epitope tag peptide YPYDVPDYA (114-122) from hemagglutinin influenza conjugated to KLH.
纯度This affinity purified Anti-HA antibody is directed against the HA motif and is useful in determining its presence in various assays. This polyclonal anti-HA tag antibody detects over-expressed proteins containing the HA epitope tag. To date, this antibody has reacted with all HA-tagged proteins tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.
偶联Unconjugated

储存与操作

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储存Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
形态/外观Liquid (sterile filtered)
缓冲液/防腐剂Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
浓度1.05 mg/mL
有效期12 months from date of receipt.
干冰运输请注意:本产品需要干冰运输,将收取干冰附加费。
免责声明For research use only

别名

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rabbit anti-HA epitope tag antibody, rabbit anti-hemagglutinin antibody, rabbit anti-HA tag antibody, anti-epitope

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质量保证

质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.

实验方案信息

WB
免疫印迹(WB)
查看实验方案
IHC
免疫组化
查看实验方案
ELISA
酶联免疫吸附测定(ELISA)
查看实验方案

可选规格

请在下方选择规格

100 μg
选择偶联或无载体版本
免费二抗(20 µl)0/0

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