HighFidelity GREEN PCR Labeling Testkit

SKU: orb692240

描述

HighFidelity GREEN PCR Labeling Testkit is designed to produce randomly Fluorescein-, ATTO488-and AF488-modified DNA probes by PCR to find the optimal label for the green emission wavelength range. Such probes are ideally suited for Fluorescence in situ hybridization (FISH) and Northern Blot experiments. PCR-based labeling is superior to random-primed labeling with Klenow fragment if template amounts are limited or amplification of a specific DNA fragments is required. Amplification of probes up to 4 kbp is feasible. All labeled-dUTPs are efficiently incorporated into DNA as sub-stitute for its natural counterpart dTTP using an optimized reaction buffer and a High Fidelity Polymerase blend consisting of Taq polymerase and a proofreading enzyme. 50% labeled-dUTP substi-tution typically results in an optimal balance between reaction and labeling efficiency. Individual optimization of labeled-dUTP/dTTP ra-tio however, can easily be achieved with the single nucleotide format.

研究领域

Molecular Biology

图片与验证

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应用说明	Spectroscopic Propertie: Fluorescein: λexc 492 nm, λem 517 nm, ε 83.0 L mmol-1 cm-1 (Tris-HCl pH 7.5), ATTO488: λexc 500 nm, λem 520 nm, ε 90.0 L mmol-1 cm-1 (Tris-HCl pH 7.5), AF488: λexc 494 nm, λem 515 nm, ε 73.0 L mmol-1 cm-1 (Tris-HCl pH 7.5).

关键属性

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储存与操作

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储存	store at -20 °C
有效期	12 months from date of receipt.
危害信息	Non-Toxic
免责声明	For research use only

别名

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Preparation of FAMX-, ATTO488- and AZDye488-labeled DNA probes by PCR

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实验方案信息

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