HighYield T7 Cy5 RNA Labeling Kit (UTP-based)

HighYield T7 Cy5 RNA Labeling Kit (UTP-based) is designed to produce randomly Cy5-modified RNA probes via in vitro transcription. Such probes are ideally suited for in situ hybridization and Northern Blot experiments. UTP-X-CY5 (also known as Cy5-17-UTP from GE Healthcare) is effi-ciently incorporated into RNA as substitute for its natural counterpart UTP using an optimized reaction buffer and T7 RNA Labeling Poly-merase Mix. 35% UTP-X-Cy5 substitution typically results in an optimal balance between reaction and labeling efficiency. Individual optimization of UTP-X-Cy5/UTP ratio however, can easily be achieved with the single nucleotide format. The resulting Cy5-modified RNA probe can subsequently be detected by fluorescence spectroscopy. The kit contains sufficient reagents for 20 labeling reactions of 20 µl each (35% UTP-X-Cy5 substitution, 2.5 mM ATP, GTP, CTP, 0.2 mM UTP, 0.1 mM UTP-X-Cy5).