Recombinant Histone H1 Antibody / Rabbit Monoclonal

SKU: orb533994

描述

Five major families of histones exist: H1/H5, H2A, H2B, H3, and H4. Histones H2A, H2B, H3 and H4 are known as the core histones, while histones H1/H5 are known as the linker histones. The core histones all exist as dimers, which are similar in that they all possess the histone fold domain; three alpha helices linked by two loops. It is this helical structure that allows for interaction between distinct dimers, particularly in a head-tail fashion (also called the handshake motif). The linker histone H1 binds the nucleosome at the entry and exit sites of the DNA, thus locking the DNA into place and allowing the formation of higher order structure. [Wiki]

研究领域

Epigenetics & Chromatin

图片与验证

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已测应用	FACS, IF, IHC-P
稀释范围	Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RTPrediluted IHC only format: incubate for 30 min at RT (1)
反应性	Human
应用说明	The stated application concentrations are suggested starting points. Titration of the recombinant Histone H1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

关键属性

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抗体类型	Primary Antibody
宿主	Rabbit
克隆性	Recombinant
同种型	Rabbit IgG, kappa
克隆号	OSHT-3R
免疫原	Recombinant full-length human protein was used as the immunogen for the recombinant Histone H1 antibody.
纯化	Protein A affinity chromatography
偶联	Unconjugated

储存与操作

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储存	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
缓冲液/防腐剂	0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
有效期	12 months from date of receipt.
免责声明	For research use only

相似产品

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Item 1 of 3
Recombinant Histone H1 Antibody / Rabbit Monoclonal [orb2640889]
应用：
FACS, IF, IHC-P
反应性：
Human
种属/宿主：
Rabbit
克隆性：
Recombinant
偶联：
Unconjugated
可选规格： 100 μg
Item 1 of 2
Recombinant Histone H1 Antibody / Rabbit Monoclonal [orb2642125]
应用：
IHC-P
反应性：
Human, Mouse, Rat
种属/宿主：
Rabbit
克隆性：
Recombinant
偶联：
Unconjugated
可选规格： 7 ml
Item 1 of 1
Recombinant Histone H1 Antibody / Rabbit Monoclonal [orb2640888]
应用：
IHC-P
反应性：
Human
种属/宿主：
Rabbit
克隆性：
Recombinant
偶联：
Unconjugated
可选规格： 7 ml

质量保证

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